normal lung epithelial cell line beas 2b (ATCC)

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ATCC normal lung epithelial cell line beas 2b

THOC3 expression is upregulated in LC and is associated with unfavorable prognoses. (A, B) The mRNA expression of THOC3 in LUAD and LUSC tissues, as well as normal lung tissues, was obtained from the GEPIA database. (C) Real-time PCR analysis of THOC3 mRNA expression in cancerous and adjacent non-cancerous tissues from LC patients. (D) Western blot analysis of THOC3 protein levels in cancerous and adjacent tissues from LC patients. (E) Real-time PCR analysis of THOC3 mRNA expression in LC cell lines (H1975, A549, H1299, and PC9) and the normal bronchial epithelial cell <t>line</t> <t>BEAS-2B</t> (F) Western blot analysis of THOC3 protein expression in LC cell lines (H1975, A549, H1299, and PC9) and BEAS-2B cells. (G) Real-time PCR analysis of THOC3 mRNA expression in normal lung tissues and LC samples from different clinical grades. (H) Western blot analysis of THOC3 protein levels in normal lung tissues and LC samples from various clinical stages. (I, J, K) Kaplan-Meier survival curves illustrating the overall survival (OS) of LC patients with high or low THOC3 expression from the TCGA database. (L) Kaplan-Meier survival curves depicting disease-free survival (DFS) in high vs. low THOC3-expressing LC patients from the TCGA database. Each experiment was performed in triplicate. Data are expressed as mean ± SEM; * P < 0.05.

Normal Lung Epithelial Cell Line Beas 2b, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4054 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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normal lung epithelial cell line beas 2b - by Bioz Stars, 2026-10

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1) Product Images from "THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway"

Article Title: THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway

Journal: Translational Oncology

doi: 10.1016/j.tranon.2026.102741

Figure Legend Snippet: THOC3 expression is upregulated in LC and is associated with unfavorable prognoses. (A, B) The mRNA expression of THOC3 in LUAD and LUSC tissues, as well as normal lung tissues, was obtained from the GEPIA database. (C) Real-time PCR analysis of THOC3 mRNA expression in cancerous and adjacent non-cancerous tissues from LC patients. (D) Western blot analysis of THOC3 protein levels in cancerous and adjacent tissues from LC patients. (E) Real-time PCR analysis of THOC3 mRNA expression in LC cell lines (H1975, A549, H1299, and PC9) and the normal bronchial epithelial cell line BEAS-2B (F) Western blot analysis of THOC3 protein expression in LC cell lines (H1975, A549, H1299, and PC9) and BEAS-2B cells. (G) Real-time PCR analysis of THOC3 mRNA expression in normal lung tissues and LC samples from different clinical grades. (H) Western blot analysis of THOC3 protein levels in normal lung tissues and LC samples from various clinical stages. (I, J, K) Kaplan-Meier survival curves illustrating the overall survival (OS) of LC patients with high or low THOC3 expression from the TCGA database. (L) Kaplan-Meier survival curves depicting disease-free survival (DFS) in high vs. low THOC3-expressing LC patients from the TCGA database. Each experiment was performed in triplicate. Data are expressed as mean ± SEM; * P < 0.05.

Techniques Used: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Expressing:

Article Title: THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway
Article Snippet: The human normal lung epithelial cell line BEAS-2B was obtained from the American Type Culture Collection.. Four human-derived non-small cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).Four human-derived non-small cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).

Article Title: THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway.
Article Snippet: The human normal lung epithelial cell line BEAS-2B was obtained from the American Type Culture Collection.. Four human-derived nonsmall cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).Four human-derived nonsmall cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).

Article Title: RecA Inhibitor Mitigates Bacterial Antibiotic Resistance
Article Snippet: The reference genome sequence NZ_CP009273.1 and its annotation (downloaded from NCBI) were used in the bioinformatics.The reference genome sequence NZ_CP009273.1 and its annotation (downloaded from NCBI) were used in the bioinformatics.. The human normal lung epithelial cell line BEAS-2B and human non-small cell lung cancer cell lines A549, H292, and H1299 used in this study were obtained from our research group and purchased from ATCC [ ].. During cell culture, strict mycoplasma testing was performed regularly to ensure that the cells were free of mycoplasma contamination.During cell culture, strict mycoplasma testing was performed regularly to ensure that the cells were free of mycoplasma contamination.

Article Title: Copper‐Containing Mesoporous Bioactive Glass Nanoparticles: A Novel Radiosensitizer for Nonsmall Cell Lung Cancer
Article Snippet: Human lung adenocarcinoma cell lines A549 and H1299, along with the human normal lung epithelial cell line BEAS-2B, were sourced from the American Type Culture Collection (ATCC, Shanghai branch, China).. A549 and H1299 cells were cultured in RPMI-1640 medium (Gibco Ltd, USA), while BEAS-2B cells were maintained in DMEM (Gibco Ltd, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Sigma Ltd, USA).A549 and H1299 cells were cultured in RPMI-1640 medium (Gibco Ltd, USA), while BEAS-2B cells were maintained in DMEM (Gibco Ltd, USA) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin (Sigma Ltd, USA).

Article Title: Combination of RNA-sequencing data analysis, network pharmacology, molecular docking techniques to investigate the mechanism of Prunella vulgaris L. in the treatment of non-small cell lung cancer
Article Snippet: Human NSCLC cell lines A549 and NCI-H1385, and immortalized normal lung epithelial cell line BEAS-2B were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).. The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO 2 .The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO 2 .

Article Title: Combination of RNA-sequencing data analysis, network pharmacology, molecular docking techniques to investigate the mechanism of Prunella vulgaris L. in the treatment of non-small cell lung cancer.
Article Snippet: Human NSCLC cell lines A549 and NCI-H1385, and immortalized normal lung epithelial cell line BEAS-2B were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).. The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO2.The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO2.

Article Title: ARPC1A Promotes NSCLC Malignancy via Stimulating the Drug Resistance and Cell Migration
Article Snippet: The human non-small cell lung cancer (NSCLC) cell lines PC9, HCC827, H1975, and H1299, along with the human normal lung epithelial cell line Beas-2B and HFL1, were purchased from the American Type Culture Collection (ATCC, USA).. PC9 and Beas-2B cell lines were maintained in Dulbecco’s modified Eagle’s medium (Pricella, PM150210, Wuhan, China) supplemented with 10% Fetal bovine serum (VivaCell Biosciences, C04001-500, Shanghai, China) and 1% Antibiotics (Biosharp, BL505A, Hefei, China), while HCC827 and H1975 cell lines were cultured in Roswell Park Memorial Institute 1640 medium (Pricella, PM150110, Wuhan, China).PC9 and Beas-2B cell lines were maintained in Dulbecco’s modified Eagle’s medium (Pricella, PM150210, Wuhan, China) supplemented with 10% Fetal bovine serum (VivaCell Biosciences, C04001-500, Shanghai, China) and 1% Antibiotics (Biosharp, BL505A, Hefei, China), while HCC827 and H1975 cell lines were cultured in Roswell Park Memorial Institute..

Real-time Polymerase Chain Reaction:

Article Title: THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway.
Article Snippet: The human normal lung epithelial cell line BEAS-2B was obtained from the American Type Culture Collection.. Four human-derived nonsmall cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).Four human-derived nonsmall cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).

Article Title: Combination of RNA-sequencing data analysis, network pharmacology, molecular docking techniques to investigate the mechanism of Prunella vulgaris L. in the treatment of non-small cell lung cancer.
Article Snippet: Human NSCLC cell lines A549 and NCI-H1385, and immortalized normal lung epithelial cell line BEAS-2B were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).. The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO2.The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO2.

Western Blot:

Article Title: THOC3 interacts with epithelial-to-mesenchymal transition to promote non-small cell lung cancer carcinoma progression through STAT3 signaling pathway.
Article Snippet: The human normal lung epithelial cell line BEAS-2B was obtained from the American Type Culture Collection.. Four human-derived nonsmall cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).Four human-derived nonsmall cell lung cancer cell lines (H1975, A549, H1299 and PC9) were acquired from the China Academia Sinica Cell Repository (Shanghai, China).

Article Title: Combination of RNA-sequencing data analysis, network pharmacology, molecular docking techniques to investigate the mechanism of Prunella vulgaris L. in the treatment of non-small cell lung cancer.
Article Snippet: Human NSCLC cell lines A549 and NCI-H1385, and immortalized normal lung epithelial cell line BEAS-2B were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA).. The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO2.The cells were placed in Petri dishes containing Dulbecco’s Modified Eagle’s Medium (DMEM; Invitrogen, Carlsbad, CA, USA), with 10% fetal bovine serum (FBS; Beyotime, Shanghai, China), 100 U/mL penicillin and 100 μg/mL streptomycin (Invitrogen, Carlsbad, CA, USA), and cultured at 37 °C in 5% CO2.

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